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For ATP hydrolysis experiments, closure was initiated with 2 mM ATP in a reaction buffer consisting of 50 mM Hepes pH 7.5, 50 mM KCl.
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(D ) Kinetic experiments measuring closure and (E ) ATP hydrolysis.
In all experiments complete closure events happened instantaneously in one step and not in e.g. three sequential steps, which would be typical for trimeric beta-barrel proteins like OmpF [ 26].
We identified seven AWP closure experiments in Australia.
The model performance was evaluated against measured data obtained from several system closure experiments.
For example, contaminating ADP is a confounding factor in fluorescence resonance energy transfer experiments measuring arm closure rates of Hsp90.
The "9C14" construct was of particular interest because footprinting, electrophoretic mobility shift, and ring closure experiments suggested that it forms both geometries.
Figure 6 Examples of loop closure experiments using visual SIFT features.
In transmigration and wound closure experiments, however, knockdown of cadherin-9 had no significant effects (data not shown).
Circular (∼1.0 2.0 mm in diameter for conventional wound closure experiments) or linear (∼0.2 mm in width for analysis of rapid cell migration) wounds were scraped in each well with a sterile 10-µl pipette tip.
Wound closure experiments to evaluate migration of MDCK cells in the presence of cucurbitacin I (both initial screening and subsequent quantitative analyses for concentration-response profiling) were carried out as previously described, as were determinations of cell viability at the end of each experiment [28].
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