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In control experiments, cell viability is verified by the addition of a two-photon fluorescence channel to the OCM.
Bivariate contour plots based on millions of observations are increasingly required in applications including high-energy physics simulation experiments, cell sorters and geographical data representation.
During all experiments, cell viability was ≥90%.
For time course experiments, cell numbers were determined daily.
For our experiments, cell populations between passage 3 and 5 were used.
In both experiments, cell proliferation was measured by assessment of tritiated thymidine incorporation.
In some experiments, cell were counted by flowcytometry using Flow-Count fluorospheres (Beckman Coulter).
For these experiments, cell density (∼30% confluence at the time of transfection) was critical for transfection efficiency.
For RNase experiments, cell extracts from no tag control or cells expressing Caf1-Myc were prepared as described above.
Before the S-WGA experiments, cell cultures were retrieved and washed once with phosphate-buffered saline (PBS).
Furthermore, in these experiments cell populations were starved for carbon prior to isolation of swarmer cells [10].
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