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The brevetoxin congener (PbTx-3 was chosen for these experiments because it is less reactive to metabolism in rodents than its precursor (PbTx-2) (Radwan et al. 2005).
Olivine was used in experiments because it is a mineral frequently found in many stony meteorites and is thus a relevant analogue for such meteorites.
ZA was chosen for testing in these experiments because it is clinically used in humans with cancer, and has been shown to modulate rat smooth muscle cell proliferation and migration.
The first step defines the independent variables of major effects on the reaction system via screening experiments, because it is not possible to identify the effects of all parameters.
The steps of RSM as an optimization method are as follows: (1) The first step defines the independent variables of major effects on the reaction system via screening experiments, because it is not possible to identify the effects of all parameters.
We selected Nd-0 for mapping experiments because it is morphologically similar to Col-0.
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Evaporation was neglected for short-term experiments because it was around 1 mm · d−1.
However, it was gradually re-mobilized in the latter part of the experiments because it was only weakly adsorbed via outer sphere complexation reactions.
Position 4 is excluded from these experiments because it was found earlier that signals at position 4 may not contribute at all or only marginally to discrimination.
The 72-h time point was chosen for these experiments because it was shown to coincide with peak blood-spinal cord barrier permeability during the acute phase of SCI (unpublished data).
TFA was not used in these initial experiments because it was not in stock.
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