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Moreover, the same experiments ascertained that secondary as opposed to primary necrosis occurred after 8 h (Fig. 2).
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In control experiments, we ascertained that rat bisulfonated genomic DNA obtained from control mammary tissue was amplified in the linear range with U- and M-specific Brca-1 oligonucleotides, and Brca-1 amplicons were of the expected size (142 bp) (Fig. 2a).
The overexpression of the active form of the N-receptor (Nintra) driven by a heat-shock promotor was used to ectopically trigger the N-pathway after a temperature pulse (HS) to 37°C for 10 min. In a series of control experiments, we ascertained that the kinetic of the N-response triggered under Nts-1 and Nintra conditions were compatible with the timing of phases of the cell cycle.
In preliminary experiments, we ascertained that the cotransfection of Hela cells with p3XERE plus an empty vector (pCR3.1) did not yield measurable luciferase activity.
This experiments ascertained the ability of these sycophagine wasps to induce galls.
This work shows that functional complementation experiments can be readily performed in order to ascertain that phenotypic effects observed upon RNAi experiments are indeed due to the specific silencing of the targetted gene.
In particular, a robust normalization method is critical to ascertain that arrays from two experiments are comparable with minimum noise prior downstream analysis.
The same set of experiments was performed with heat-inactivated S30 extracts to ascertain that degradation is dependent on components present in the Sso extracts.
To ascertain that selective inhibitors tested in FRAP experiments do not cross-react with other bromodomains in the cellular environment, the BET inhibitor PFI-1 was profiled across 10 bromodomain targets in FRAP assays at 1 μM.
In our experiment, we ascertained that our samples were novice learners (i.e., they did not have prior knowledge regarding subject domain).
To ascertain that variations of chromatin flexibility do not necessarily impact higher-order chromatin dynamics, we performed quantitative 3C experiments in mESC that are Triple Knock-Out (H1 TKO) for histone H1 genes H1c, H1d and H1e [ 22].
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