This validation experiment ascertains the effect of removing any word by presenting the text with no time pressure and asking subjects to determine whether the sentence is true or false from the information given.
The overexpression of the active form of the N-receptor (Nintra) driven by a heat-shock promotor was used to ectopically trigger the N-pathway after a temperature pulse (HS) to 37°C for 10 min. In a series of control experiments, we ascertained that the kinetic of the N-response triggered under Nts-1 and Nintra conditions were compatible with the timing of phases of the cell cycle.
In preliminary experiments, we ascertained that the cotransfection of Hela cells with p3XERE plus an empty vector (pCR3.1) did not yield measurable luciferase activity.
Full activation of the cofactor molecules prior to use in various experiments was ascertained by immunoblotting with both a mixture of monoclonal antibodies to factor V heavy and light chains (provided by K. G. Mann from the Department of Biochemistry, University of Vermont, Burlington, VT) and a polyclonal antibody to factor V made in sheep.
In control experiments, we ascertained that rat bisulfonated genomic DNA obtained from control mammary tissue was amplified in the linear range with U- and M-specific Brca-1 oligonucleotides, and Brca-1 amplicons were of the expected size (142 bp) (Fig. 2a).
In the present experiments, we ascertained from optical imaging, with electrophysiological corroboration, that our injections corresponded to regions responsive to objects or to body-including-faces of animals and humans, but not specifically to face or color stimuli (Supplementary Figs 1 and 2).
In our experiment, we ascertained that our samples were novice learners (i.e., they did not have prior knowledge regarding subject domain).
Quality of the experiment was ascertained by the mad1.dr value (medium absolute deviation of the log 2 ratio difference between consecutive probes) that provides a surrogate measure of experimental noise and should be <0.23.
The entire study was conducted in three phases:(a) screening experiments, to identify process variables that critically influence taper in laser drilled holes, (b) Optimization experiments, to ascertain the set of parameters that would yield minimum taper and (c) validation trials, to assess the validity of the experimental procedures and results.
