Many experiments were setup, however, in this article, we include the more relevant results.
In addition field experiments were setup to determine loading time and transmission rate.
Experiments were setup as double staining where all samples were stained with Hoechst (Invitrogen, USA) to detect nuclei.
Two different flume mesocosm experiments were setup to test for: i) the effect of membranes on burial of Z. marina seeds; ii) the effect of membranes on survival and growth of Z. marina seedlings.
Control binding experiments were setup using the LightShift EMSA optimization and control kit (Thermo).
Crystallization experiments were setup using the hanging-drop vapor diffusion method, by mixing protein (∼7 mg/ml) and well solutions (1 1 vol:vol), and incubated at 4°C.
The time course analysis of this set of experiments was setup somewhat differently than in the CD3+ T-cell study (0, 6, 12, 24, 48 and 72 hours in the CD4+ and CD8+ subsets compared to 0, 4, 10, 48, and 96 hours in CD3+ T cells) in order to cover earlier timepoints.
The optimum match condition for the 27Al{1H}CP-MAS NMR experiment was setup on Al(OH 3.
The experiment was setup with sacrificial bottles in triplicate for each time point (0, 2, 24, 48 h, 7, 14 and 28 days).
An experiment was setup to study the water film/air counter-current flow heat transfer on a vertical plate with back heating.
