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Because experiments in a meta-analysis are weighted by sample size, the huge N of these online experiments substantially lowers the mean effect size of the replications: When the online experiments are removed, the mean ES for this protocol rises to 0.06 [0.00, 0.12]; z = 1.95, p =.05.
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During statistical analysis, one of duplicated experiments was removed to avoid redundant presentation of same sample.
Since, the enriched genes from the controls likely confer general growth advantage through metabolic enhancements, any gene enriched in the n-butanol-challenged libraries that was also enriched in the control experiments was removed from further analysis.
Associations that where obtained from large scale experiments were removed.
Furthermore, metabolites detected only once in the 13 remaining experiments were removed.
Samples from aggregation experiments were removed at regular intervals and cleared by high-speed centrifugation to remove the polymeric structures.
Aliquots from the kinetic experiments were removed at the end of each experiment, and the spectra were recorded.
Unexpressed probes (p > 0.01 in all experiments) were removed from further analysis, and 15725 probes remained in the analysis.
The genes with missing values for more than one of the experiments were removed for further analysis.
Following 10 days of culturing, non-adherent DCs for use in experiments were removed from cultures by vigorous pipetting and used for experiments after DC purity was insured by FACS.
Aliquots from the kinetic experiments were removed at the end of each experiment, and the spectra were recorded as the average of three repeats over a range of 190 260 nm, at 1 nm intervals.
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