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This study focuses on this second issue and comprises two parts: field experiments and control experiments in the laboratory.
However, the µG experiments and control studies were done at exactly the same time and under the same conditions, except for the use of the 3D clinostat (Fig. 1a, arrow).
The lymphoblast cell line K562 shows minor differences in cell growth between APITD1A mRNA experiments and control mRNA experiments.
Fold differences were calculated between experiments and control counts using the quantitative data obtained from the NanoString nCounter.
Protein lysates from co-culture experiments and control cells were obtained after 7-day incubation using a ReadyPrep Sequential Extraction Kit (Bio-Rad).
Our fibroblast dataset was compared to our emphysema dataset by using a gene set created from the 100 most upregulated miR-638 predicted targets (by fold change) between miR-638 inhibitor experiments and control experiments.
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Viability reached proportional levels in all experiments and controls, compatible with the growth stage.
However, with carefully designed experiments and controls, it may be possible to overcome this problem.
We further investigate the minimal set of experiments and controls required for hands-on experiments feasible in well-equipped standard research laboratories.
Experiments and controls were replicated 6 to 10 times.
Our results provided extra value because the experiments and controls were carefully performed with concentrations of proteins that can accurately generate KD values in the range we described.
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