For all experiments age-matched FVB/N mice were used.
Twelve right-handed native English speakers with no known hearing or language impairments participated in the experiment (aged 18 38, mean age 25, 3 males).
In both experiments, age and RSI effects on PES did not interact.
In all experiments, age and sex matched mice were used at four to eight weeks of age.
Males 6 10 weeks of age were used in experiments, age and weight matched for each individual experiment to have an age span of maximum 2 weeks and a weight range of maximum ±20% of medians.
For the BM transplantation experiments, age matched wild type and Id1-/ mice were lethally irradiated (10 Gy) and reconstituted with 5000 sorted Lin- cKit+ Sca-1+ Flk-1 HSCs, along with 5×105 syngenic Id1-/ total BM mononuclear cells.
Eighteen healthy male undergraduate students, who were naïve as to the purpose of the study, were recruited for the experiments (age 22.2±0.5 years, mean±SD), on the basis of written informed consent.
Consequently, in the experiments, age-matched controls had to be used from parallel breedings.
In these experiments, age-matched cohorts of fully immunocompetent TCRα +/– and C57BL/6 wild-type mice were included for comparison.
In most experiments, age was determined by calcofluor (Sigma) staining of bud scars by including 5 μg ml−1 calcofluor in the last wash step before imaging.
