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In the Juliusruh and Andenes experiments acquisition parameters were selected to acquire data at the highest possible rate with an optimum time resolution to locate as many meteors as possible as well as to determine the high entry velocities of the Leonid meteors.
The resolution of these images was compatible with in vivo experiments (acquisition time was 1 min 36 s, compatible with anaesthetised animal's stay in the magnet) and can also depict the hypointensity related to pellets containing the lowest number of cells (0.125 × 106).
In this set of experiments, acquisition of images took 5 minutes and generated accuracy of detection and quantification.
XX designed and performed experiments, acquisition and analysis of data, and drafted the manuscript.
WD, performance of experiments, acquisition of data, interpretation of data, experimental support.
CTW designed and performed experiments, acquisition and analysis of data, and wrote the manuscript.
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LAM, AY and HAF carried out staining and in vitro experiment acquisition and analysis.
WZ: Participated in research design and animal experiment, acquisition of data and involved in drafting the manuscript.
Reducing the power of trapping beam leads to significant diminution of Raman signal-to-noise ratio, extensive averaging at the expense of single experiment acquisition time is required.
We also would like to point out that the signal to noise ratio in FCS is proportional to the fluorescence count rate per molecule times the square root of the total experiment acquisition time [ 16].
The first and second experiments, with acquisition access periods on untreated source plants, were designed to mimic primary infections due to leafhoppers coming from outside the field.
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