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For most of the experiments a concentration of 1 1015 was used because it results in single molecules separated by several μm, whereas the 1 1014 diluted sample shows a too dense concentration for our automated peak-finding routine.
In these experiments a concentration of 100 µg/ml 2-AP was used to ensure visualisation of chromosome fragmentation in the light of the experience of Cromie and Leach [7].
For subsequent experiments, a concentration of 1 ng/mL LIF was used.
In the subsequent experiments, a concentration of 12.5 μg/ml cisplatin was used.
In most of the experiments a concentration of 100 ng TNFα/ml was used.
In the experiments, a concentration of 7.9 ± 2.3 particles per cell was observed (see Fig. 3(a) ).
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For a typical experiment, a concentration of 60 × 10−6 m was used for each polymer-bound guest and CB[8] to allow formation of the ternary 1 1 1 complex.
A mixed steroid hormone working solution was prepared prior to the experiments at a concentration of 200 mg L-1 by dilution in MeOH of individual stock solutions aliquots for spiking solutions at the desired concentrations.
Isotype- and subclass-matched mouse Ig were used as negative controls in all the experiments at a concentration of 100 ng/mL.
Staurosporine was used as a positive control for apoptosis and ZO-1 rearrangement experiments at a concentration of 0.5 μM.
Following these screening assays, LACE extract was used in further experiments at a concentration of 250 μg/ml.
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