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A.M. carried out animal experimentation, sample analysis, statistical analysis and wrote the manuscript.
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T2-edited and diffusion-edited NMR spectra of CFM (collected in 2010, during the first year of experimentation) and BFM (collected in 2011, during the second year of experimentation) samples were compared to evaluate the short-medium effect of the two diets on buffalo RM composition.
YB conducted field experimentation and sample collection.
There was lot of wild horn parts and experimentation with samples.
During a 2-year-long experimentation, milk samples were collected from the same 24 buffalo animals.
Animal holding, experimentation and sampling were conducted by Dr. Martine Perret and the MECADEV team (Mecanismes Adaptatifs et Evolution, Department of Ecology and Management of Biodiversity) as described previously [40,41].
Standard procedures for animal holding, experimentation and sampling were conducted by Dr. Martine Perret and the Adaptive Mechanisms and Evolution team (MECADEV-Mecanismes Adaptatifs et Evolution, Department of Ecology and Management of Biodiversity) as previously described [2,54].
The hybridization scheme which can be defined as dye-switch was chosen to minimize the number of slides used and to test the impact of several factors on the results (conditions, labeling protocol, networks, day of experimentation and samples).
After experimentation, tissue samples of 150 μm thickness taken exactly from the injection place were cut by VT 1000S Leica vibratome and imaged with a SZX16 Olympus stereoscope in dark field mode.
Standard procedures for holding, experimentation and sampling of gray mouse lemurs were used and all animal experiments were conducted by Dr. Martine Perret and the MECADEV team (Mecanismes Adaptatifs et Evolution, Department of Ecology and Management of Biodiversity) as described by Giroud et al. [3] and presented in detail by Biggar and his colleagues [42].
Sonically, the mix is an appetizer to the Hiperasia main dish, with Díaz-Reixaemploying a lot of the same sample experimentation that he explored on the new LP.
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