Exact(1)
Experimentally, directed evolution relies upon iterative rounds of creation of novel protein variants by introduction of random mutations into the target gene and selection of individuals with desirable characteristics.
Similar(59)
The study focuses on thin surfaces with low thermal capacity to illustrate experimentally direct links between characteristics of surface thermal fluctuations and momentum-based turbulent eddy residence times.
Discovering new structural folds experimentally by directed evolution and random recombination of secondary-structure blocks is also proved rarely successful.
Furthermore, we added experimentally confirmed directed protein-DNA interactions between transcription factors and proteins.
To further demonstrate the specificity of pAbCXCR7 for the CXCR7 protein, LNCaP cells were transfected with two experimentally validated siRNAs directed against CXCR7.
Rab14 is an experimentally validated direct target of miR-451 and has been shown to activate tumour suppression [ 37]. miR451 was downregulated and the Rab14 protein upregulated (while mRNA expression remained constant) in fast growing CHO cells.
To eliminate false protein protein interactions in the pathway, only experimentally observed direct inhibitions and direct activations were considered.
To test B-SIFT's ability to predict activating mutations, we turned to the SWISS-PROT protein database, which contains literature-curated entries of experimentally determined phenotypes for directed mutagenesis experiments across a large number of proteins [7] (SWISS-PROT MUTAGEN field).
Self-similarity is observed experimentally via direct photography and documented thoroughly by direct numerical simulation of steady axisymmetric coflow flames of methane and air at pressures from 1 to 12 atm.
When investigating the dynamics of three-dimensional multi-body biomechanical systems it is often difficult to derive spatiotemporally directed predictions regarding experimentally induced effects.
DNA from skin biopsy samples and Vero and BHK 21 cells experimentally infected by using primers directed against domain I of the 23S gene of the family Waddliaceae yielded PCR products of the expected size (627 bp) (8 ).
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