Exact(5)
Using experimentally amplified sequences as queries, we performed t-blastx searches against the database of GenBank in NCBI (http://www.ncbi.nlm.nih.gov/) to confirm that there was only one significant hit for each genetic marker, thus avoiding potentially paralogous comparisons [33].
Therefore, we experimentally amplified the two regions from the corresponding species and used them in further analyses.
Third, when we used the experimentally amplified sequences as a query to search other genomes using BLAT or BLAST programs, all of these produced a single hit in the expected locations.
Between the 11 computer predicted fragments (lane 6) and 10 experimentally amplified fragments (lane 5), eight fragments matched within the length range of 100 1500 bases (Figure 4).
We performed qRT-PCR for genes in the core 8p11-12 amplinicatiourin our experimentally amplified clones (Fig. 5).
Similar(55)
"If you can experimentally amplify the memory-reinforcing process by forcing those sounds back into the brain while we're asleep," Dr. Walker said, it "may actually give us some clues as to what that mechanism is".
Use of universal adapters and indexed amplification primers offers the option to save part of the adapter-ligated DNA sample and, if experimentally necessary, amplify a library with an alternative barcode.
The value of such an experimentally tractable system is amplified by the multiple examples, described above, in which the effects of a given cannabinoid or a given change in TRP channel activity can produce distinct outcomes, depending on the biological and pharmacological circumstances.
We now report the use of that resource to identify 17483 pairs of primers that have been experimentally verified to amplify unique sequences corresponding to distinct murine transcripts.
To experimentally test their validity, we amplified twelve of these introns in a panel of six mammalian species.
First we show analytically and experimentally that amplify and forward protocols are unaffected by carrier frequency offsets at the relay.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com