Experimentally altering the physiological state of the fish differentially affected expression of the various reference genes depending on experimental design and tissue type, with 18S unaffected by the experimental treatment in all tissues examined.
One can therefore experimentally alter the specific binding site or its competing sites, as well as the transcription factor, by point mutations and then observe the effect on specificity, e.g. by measuring the expression of upstream genes.
We investigated the distribution of O-GlcNAc-modified proteins in cultured forebrain neurons and determined the effects of experimentally altering levels of O-GlcNAc on primary neuronal development.
We found that we were able to experimentally alter the CHC profile of virgin females through direct intersexual contact that mimicked the tactile courtship of this species and altered the relative abundance of several hydrocarbons.
However, the advancement of the ACA toxicity theory has been stalled due to the lack of suitable models to experimentally alter blood ACA and the technical constraints of ACA manipulation such as low boiling point and high chemical reactivity.
