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One milliliter of samples were taken after 30 min from the experimental tubes, added to ice cold PBS and centrifuged.
The percentage inhibition of superoxide generation was evaluated by comparing the absorbance values of the control and experimental tubes.
The colonisation rates in the upper 5 cm of our experimental tubes(max.
Furthermore, a small though significant differentiation in nematode assemblages was found among the experimental tubes according to depth in the sediment (R = 0.588, p = 0.008).
This procedure produced thin frozen slices that could be easily introduced in the experimental tubes while even distribution of the food source was ensured.
A total of 15 experimental tubes were prepared: threecontroltubes contained baresediment, and 4-times threetubes contained sediment with one of the initially frozen13C-labelled organisms added on top.
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Fig. 1 Diagram of first experimental tube.
The two-phase flow in the experimental tube was modeled using commercial CFD code, Fluent 6.2.
40 sets of dust dispersion system were used to disperse flake aluminum into the experimental tube.
The mist/dust/air multiphase mixtures were generated by injecting liquid/dust samples into the experimental tube.
A robust experimental tube spinning system was designed and manufactured, in which the preform and mandrel can be excited by ultrasonic vibrations.
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