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Prior to wider pharmacological validation of this approach, the variability of each quantifiable parameter was assessed over the experimental timeframe.
A total flux was calculated as area between flux curve over the indicated experimental timeframe (25 min) and the starting flux value.
Within the experimental timeframe, almost all induced genes were induced by 4 h post-exposure.
Furthermore, relative levels of bI4 maturase protein remained at least two-times higher during the entire experimental timeframe.
In our simulations (Fig. 3), we assume that the system reaches a steady state within the experimental timeframe of 2 hours.
This finding indicates either a long intracellular half-life for SMAD1 in USSC, unaltered by miRNA transfection in the experimental timeframe, or secondary regulatory mechanisms such as increased transcription that keep SMAD1 levels constant.
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Based on the results of a common-garden experimental protocol, this timeframe was sufficient to allow for population differences to emerge in plastic responses of several early-life traits to temperature (Haugen and Vøllestad 2000).
This postprandial timeframe for the experimental protocol was based on previous studies in rainbow trout, that had revealed a postprandial increase in hepatic expression of omy-miRNA-122b isomiRNA in rainbow trout 4 h after feeding [ 14], in addition to activation of the hepatic insulin pathway and regulation of metabolic gene expression.
Participants are presented with a series of neutral static images taken from the stressful film and are then asked to record the number of intrusions experienced under varying experimental conditions within a set timeframe (e.g., Holmes, James et al. 2009; Holmes et al., 2010; Lang, Moulds, & Holmes, 2009).
In contrast with previous results, we found no change in mtDNA copy number and CS activity in NR- or PARPi-treated versus untreated animals, possibly because of the shorter timeframe of our experimental protocol (4 weeks) compared to that of other studies (12 weeks).
In this study we have developed a scheme to maximize the coverage of a proteomic study of the 'shotgun approach' in a reasonable timeframe and number of experimental steps.
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