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To assess the significance of finite-aspect ratio effects and interpret experimental retention results, an ideal, analytical retention theory is needed.
Predicted and experimental retention times for 133 data points agreed to within 0.08 min and percentage normalised differences between the predicted and experimental data averaged 0.29% with a standard deviation of 0.29%.
The experimental retention time distribution curves (RTD curves) determined from tracer experiments performed at different times during the first year of operation of two field-scale steel slag filters were analyzed through a three stage process.
The experiments were performed according to the face-centred cube central composite design and the inverse form of the experimental retention times for analytes were fitted to the polynomial models.
Predicted retention times of five representative compounds on nine reversed-phase columns were compared with known experimental retention data for these columns and this comparison showed that the accuracy of the proposed modelling approach is sufficient to reliably predict the retention times of analytes based only on their chemical structures.
An increase in the aspect ratio resulted in a experimental retention time that was closer to the theoretical retention time, and exhibited higher plug flow and lower dead volume ratios compared to an ideal plug flow system operated under similar conditions.
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The highest reaction rate was obtained when the experimental hydraulic retention time (HRT) was analogous to the theoretical HRT.
HPLC optimization strategy consists of four elements; experimental design, retention modeling, quality criteria function, and optimum search method.
Results are compared with previous formulations by Brooks and Corey (1964), van Genuchten (1980) and Kosugi (1996), as well as with experimental water retention and hydraulic conductivity data of four soils having widely different soil textures.
The reliability of this model was tested using experimental chromatographic retention times as well as information about the structural characteristics of the different charge variants, e.g. C-terminal lysine groups and deamidated groups.
Hence, several different types of gradients were generated, showing a good agreement between simulated and experimental data, with retention time errors lower than 5% in most cases.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com