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For the phasing step, experimental phasing methods involving heavy atom soaks were required for the first few structures, but with the large number of serpin structures now available, molecular replacement has become the method of choice.
This crystal structure was determined using another rationally designed feature, a metal-binding site that permitted experimental phasing of the X-ray data.
At that time, there was no fancy software capable of computing the phasing, electron density modification and automatic model building, poly-peptide tracing on the density map from the experimental phasing was always a challenge.
We have determined the crystal structure of the isolated C-terminal domain of SpoVT at 1.5 Å by experimental phasing techniques and used this model to solve the structure of the full-length SpoVT at 2.6 Å by molecular replacement.
Experimental phasing was performed using PHASER EP from the CCP4 suite [44].
Experimental phasing of genotypes across 3.8 kb for rs9399137, rs9402685, and rs11759553 created unequivocal haplotype signatures for each of the patients.
Similar(36)
All experimental phases occurred in a single session in Experiment 1.
It was an experimental phase.
"But we are still in an experimental phase.
The ice creams, ominously called Dairylike, were unfortunate creations, still in the experimental phase.
It's still in a somewhat experimental phase".
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