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However, the threshold of the oscillations was significantly lower in Experimental neurons.
Experimental neurons had a lower RMP, and inward and outward rectification occurred at more hyperpolarized potentials.
However, Experimental neurons discharged more during depolarization, and some of them fired spontaneously despite having a lower threshold for outward rectification and a more hyperpolarized RMP.
Figure 4A shows examples of Control and Experimental neurons held at different membrane potentials to illustrate the lower threshold and greater amplitude of the rapid oscillations in the Experimental group.
Experimental neurons were cotransfected with 7 μg of rat O-GlcNAcase cloned in the pcDNA3.1 vector and 3 μg DsRed plasmid, control neurons were transfected with 3 μg DsRed plasmid alone (Clontech, Palo Alto, CA).
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From the experimental standpoint, neuron-to-neuron α-syn transmission has been demonstrated in vitro in a variety of cell culture systems as well as in vivo in animal models [ 3- 10].
A distributed account would predict that in other experimental paradigms, single neurons would show similar competitive dynamics to our DLPFC neurons but in complementary frames of reference (Rustichini and Padoa-Schioppa, 2015; Strait et al., 2014).
This matches experimental observations in neurons of the pancreas of mice (Xu et al., 2006) and molluscan neurons as well as previous simulations of an extended Hodgkin–Huxley model (Rush and Rinzel, 1995) and is consistent with the effect of peak conductances on firing rate, for example (Schreiber et al., 2004).
Other examples in the literature suggest that, under certain experimental conditions, serotonergic neurons can release DA.
Following experimental treatment, cultured neurons were fixed with fixative solution (10 mL 4% paraformaldehyde plus 1.5 mL saturated picric acid) for 10 min at room temperature, following by fixation with methanol for 10 min at −20°C.
In contrast, under identical experimental conditions, three neurons with an SA-mechanosensitive current were unaffected by local subtilisin treatment.
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