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Unfortunately, the experimental maneuvers associated with the coverslip installation in these chambers (medium removal and re-application) trigger unintended [Ca2+]i elevations.
All mice were handled daily to habituate them to experimental maneuvers.
When used to infer tension, this assay assumes that the frictional elements (which would reflect the viscoelastic properties of the junctions) do not change between experimental maneuvers.
If the suppression of glucose-evoked insulin secretion from intact pancreatic islets also results from this effect, then experimental maneuvers that expand the boundaries of [Ca2+]i microdomains should rescue insulin secretion.
The differences in the results of the two studies might represent a species difference, a postnatal age effect, an anesthesia effect, or some other detail of the experimental maneuvers.
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Design of clinical research whose purpose is to answer questions about causality can be classified in relation to four axes: the number of study groups, the implementation of an experimental maneuver, cause-effect directionality and source from which the data are collected.
To further confirm these effects, we measured the phosphotyrosine protein phosphorylation profile and levels of filamentous (F) and globular (G) actin in HBSM cells subjected to transient stretch; over the short duration of the experimental maneuver, the total amount of actin must be conserved.
Using a new experimental maneuver that permits clear and correct isolation of SCs from the fiber of origin, we report, for the first time, that two subpopulations of SCs coexist in fixed proportions on the single fiber: the low proliferative (LPC) and the high proliferative clones (HPC) which show alternative myogenic potential in vitro retained also in vivo.
Changes in [Ca]i were calculated by determining the rise in [Ca]i relative to basal levels measured immediately before that particular experimental maneuver.
This experimental maneuver was previously demonstrated to partially rescue the delayed asynchronous GABA release in SynII−/− DG neurons (Medrihan et al. 2013).
The tracking control is validated in experimental swing up maneuvers of the pendubot.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com