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It is also noteworthy that the mean departure latency of the whole group did not vary with the experimental group size.
First, we suggest conducting studies with larger experimental group size, and evaluation over a greater time frame.
Using the values of the placebo event rate of 0.11 (9/83) and the paracetamol/codeine event rate of 0.57 (65/114), we simulated 10,000 trials with experimental group size of 114 and a control group size of 83 and counted the proportion in which the simulated NNT was within ± 0.5 of the true NNT of 1.9 [ 11].
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Experimental group sizes were determined by a power calculation to detect differences at the 0.05 level.
Such improvements would meet highest ethical standards, even if individual experimental group sizes might be bigger.
experimental group sizes are limited, and models are needed, even in well controlled experiments, to distinguish random variation from true biological effects.
This will require a commitment among RTT researchers to fully disclose all aspects of study design in their publications, including (but not limited to) allocation concealment, blinded assessment of outcome, random allocation of subjects to experimental groups, experimental group sizes, breeding strategies and statistical methods.
However, actual experimental group sizes were reduced to n = 7 due to one animal not receiving pre-operative aspirin treatment and one animal not being able to receive a double ovarian autotransplant due to damage to the uterine artery during initial surgery (Fig. 1).
An important advantage of this approach for longitudinal evaluation of the same retinal neurons is that it avoids the uncertainty associated with having to compensate for the normal large variation in RGC numbers among individual mice by using large experimental group sizes for each time point.
For all experimental groups, group size was ≥6.
Seventy-two extracted human third molars, assigned to experimental groups by size, received standardized tooth preparation.
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