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Based on this promising experimental groundwork we hypothesized that we can screen for stabilized IgG1-Fc variants by random mutagenesis of the Fc-gene, followed by heat incubation of the Fc-library displayed on the yeast surface, flow-cytometric sorting of yeast cells displaying folded Fc-protein and plasmid DNA isolation from the sorted cells.
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In this exploration of Elizabethan London's burgeoning scientific community, Harkness demonstrates how an assortment of barber-surgeons, midwives, and alchemists who, along with hundreds of laymen and women, took a "vernacular approach to experimental practice" laid the groundwork for the scientific revolution of the late sixteenth and seventeenth centuries.
During the first half of the 20th century, when much of the groundwork in experimental psychology was laid, ethologists (who were for the most part European) concerned themselves with behaviour in insects, fishes, and birds and were particularly interested in the evolution of instinct.
Thus, a theory of motivational scaffolding lays the groundwork for experimental research testing motivational theories by investigating the predicted effects of motivational scaffolds.
This experimental investigation performs the necessary groundwork and builds a strong foundation towards making driving on ice safe.
His curiosity, about compasses and lodestones, led this 16th century physician beyond his chosen field and into physics, where his careful explanation of his observations and reasoning laid the groundwork for modern experimental science.
"We haven't got that kind of power, so what's the point of calling it an experimental zone?" asks Mr Pu.The groundwork, at least, for more radical change is at last being laid.
Our results explain recent experimental findings and lay the groundwork for studying the hierarchical molecular mechanisms that underpin the thermomechanical stability/instability of coiled coils and similar protein assemblies.
The molecular phenotype described provides groundwork for future experimental approaches aimed at identifying functionally important maternal transcripts and proteins involved in oogenesis and early stages of embryo development.
The molecular phenotype described provides groundwork for future experimental approaches aimed at identifying functionally important stored maternal transcripts and proteins involved in oogenesis and early stages of embryo development.
A pilot plant fed with municipal raw wastewater was operated in order to obtain experimental results supporting the developed algorithm groundwork.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com