We gratefully acknowledge J McNicol, C Hackett and D Roberts for valuable discussions concerning the experimental and custom array design; F Yeo, A Gonzalez, Z Kohutova, F Meijer-Dekens, R Aghnoum, M Macaulay and K McLean for their kind help with sampling; and Drs A Newton and G Bryan for their critical review of the manuscript.
While industrially manufactured oligonucleotide arrays (e.g. Agilent Whole Genome 44 k Oligoarray and CodeLink Whole Genome Bioarray) have optimized protocols that perform well on both single and dual-channel microarray experimental designs, custom made spotted cDNA or oligonucleotide microarrays have been mainly employed in two-channel designs [ 1].
where he creates experimental and custom-designed products, mostly related to bicycles.
Nowadays, Data Analysis in High Energy Physics (HEP) means handling billions of experimental data in custom software frameworks.
I conclude that an emphasis on custom experimental designs is key to continued empirical progress in the field.
Due to the high accuracy required to measure the few-part-per-million asymmetry, the G0 experiment was based on a custom experimental setup with its own associated electronics and data acquisition (DAQ) system.
Experiments have been carried out by means of Differential Scanning Calorimetry (DSC) and by two other custom experimental procedures designed by the authors, in order to ensure that, the small samples tested in the DSC and the large samples tested using those experimental techniques, undergo the same or equivalent thermal processes.
Our experimental design and custom scripts, however, are flexible to meet virtually all experimental control and data acquisition needs of those interested in conducting behaviorally controlled, response-based experiments.
Subjects sat in front of a custom experimental device, comprising a high speed cathode-ray tube (CRT) screen (frequency: 160 Hz) and a 21-in screen (ViSaGe, Cambridge Research Systems, Rochester, UK).
To determine the optimal length of DMF feeding for induction of NQO1 activity, CD-1 nude mice were implanted subcutaneously in the right flanks with 5 × 10 Hcells cells and the mice were fed custom experimental diet containing no DMF for 18, 15, 11 or 4 days.
