We verified the consistency of the chromosome whole assembly by comparing the genome sequence map with a genetic experimental map obtained by conjugation [22].
Experimental identifications of ubiquitin-conjugation sites on ubiquitylated proteins in vivo and in vitro are the foundation for understanding the mechanisms of ubiquitination dynamics.
We next tested the accuracy of our assay in profiling SUMO1 and NEDD8 conjugation activities using similar experimental strategies.
In this work we present, for the first time, experimental evidence that P. falciparum utilizes SUMO conjugation to modify blood stage proteins.
The model is applied to an experimentally characterized microstructure, i.e. conjugation boundary, and the predicted mechanical response is verified by comparison to experimental measurements.
Extended Experimental Procedures Bacterial Strains, Growth Conditions, and Conjugations All E. coli strains used in this study (Table S3 ) were grown in LB medium under aeration at 37°C.
Experimental details on MNP synthesis, surface modification, cDNA conjugation, and surface passivation as well as detailed assay procedures and supporting figures.
It is demonstrated, on an experimental model of human pancreatic adenocarcinoma, that lipid conjugation of Met-Enk enhances its tumor suppression efficacy compared to its nonlipidated counterparts, both in vitro and in vivo.
The mathematics so far used to describe the kinetics of conjugation in biofilms are developed largely from experimental observations of planktonic gene transfer, and are absent of lags or plasmid stability that appear experimentally.
These examples, as well as numerous genes annotated as "hypothetical" found to be differentially represented among the conjugation EST set, are promising candidates for experimental analysis, using the tools of Tetrahymena reverse genetics [ 1, 89].
