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Since this PIV experiment was executed at low Reynolds number, the Reynolds number of inflow turbulence numerically obtained was also low.
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Each experiment was executed as a randomized complete block design with three replicates.
An herbivore exclusion experiment was conducted at the site Buoy 0 (Figure 1) and a nutrient enrichment experiment was conducted near the Water Factory, located 2.5 km east of Buoy 0. Nutrient and herbivore exclusion experiments were executed at different locations to avoid crowding and interference between different experimental manipulations.
All experiments were executed at room temperature (24°C).
Therefore in the current study, enzymatic hydrolysis experiments are executed at 20% solids in roller bottles to provide the data required for future techno-economic analysis.
The experiments were executed at 50°C, using 30% DM of straw supplemented with buffer (phosphate-citrate, 50 mM, pH 5,5) and an enzyme mixture of 5 FPU per g DM, consisting of Celluclast 1.5 L and Novozyme 188 (both from Novozymes A/S, Bagsværd, Denmark) in ratio of 5 1 v/v.
Finally, the experiment was executed.
After the training, the pilot experiment was executed.
The experiment was executed in triplicate for accuracy.
The first experiment was executed approximately one year before the second and third experiments.
The ground control experiment was executed analogously to the flight scenario.
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