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Jatropha curcas (KUBP 78-9 Var)., having been planted at 2 × 2 m spacing, was aged 2 yr when the experiment was commenced.
The experiment was commenced at 6 a.m. of a day and those were completed at 6 a.m. of the next day to observe the daily feeding rate.
Before the formal experiment was commenced, the subject went through sufficient training so that the thresholds could be stabilized and proper intervals for the 7 orientations of the test line could be empirically determined.
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The animals were allowed between 7 10 days to recover from surgery before experiments were commenced.
Experiments were commenced 7 10 days after surgery.
PCOS induction experiments were commenced at ∼28 days.
An equilibration time of 15 min was used before experiments were commenced.
All experiments were commenced only when the mortality rate of zebrafish was <2% for at least one week.
All experiments were commenced between 8 00 and 10 00 am, and followed during 20 days after tumor implantation.
SSF experiments were commenced by adding spore suspension (1 × 10 spore/g of dry wheat straw) into each Duran bottle.
Before synaptic plasticity experiments were commenced, all animals were first assessed in a baseline experiment, where the stability of potentials evoked by test pulses (0.025 Hz) was assessed.
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