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This experiment was adapted from that described in a previous report after several modifications (Beardslee et al. 2014).
This was observed for every experiment when multiple injections were made (data not shown); thus the experiment was adapted to include only a single injection of MMC.
The assay used in this experiment was adapted from Roos et al. and Trouw et al.. Considering the difference in coating substrate and antibodies, various concentrations of all the reagents were tested in order to optimize the assay.
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The procedure for reverse transcription quantitative PCR experiments was adapted from (Castanera et al. 2015).
An in-house script, finalList.pl, described previously [47] for applying parsimony (Occam's razor) to generate a non-redundant list of identified proteins from a large pool of independent experiments was adapted to simultaneously calculate average peptide ratios for each protein in each block.
An in-house script, finalList.pl (available here: http://www.chibi.ubc.ca/wp-content/uploads/2013/01/finalList.pl_.txt) for applying parsimony (Occam's razor) to generate a non-redundant list of identified proteins from a large pool of independent experiments was adapted to simultaneously calculate average peptide ratios for each protein in each block.
The Internet activities used for these experiments were adapted from an abundance of information to include past experience and knowledge.
Immunoprecipitation experiments were adapted from31.
The generated basis functions of the finger-related experiments are adapted from real data to validate the incorporation of non-delayed and real-time event-related features and to effectively demonstrate a dynamic-modeling-based online framework.
Flow cytometry experiments were adapted from Combes and colleagues [ 14] and Robert and colleagues [ 15].
Blue native gel electrophoresis experiments were adapted from Schagger and Von Jagow [ 28].
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