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To start the experiment, viable cells were counted using trypan blue stain and were diluted to 1×105 cells/ml into fresh medium.
During the flow cytometry experiment, viable cells were selected on the basis of high NADH autofluorescence and low fluorescence from the cell-impermeable nucleic acid stain Sytox Green.
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Modern software engineering demands professionals and researchers to proactively and collectively work towards exploring and experimenting viable and valuable mechanisms in order to extract all kinds of degenerative bugs, security holes, and possible deviations at the initial stage.
In all experiments viable cells were gated based on forward and side scatter profiles and dead cells were further excluded using Propidium iodide.
In some experiments, viable cells (attached and floating) were detected by Trypan blue dye exclusion after UVC exposure.
ENEA has conceived an in-pile testing (IFA-652 experiment) of viable inert matrices concepts: CSZ and thoria.
Thus, a robust on-line blend monitoring method can be fully developed before any large-scale blending experiment is viable, allowing the blend method to be used during scale-up and blend development trials.
The experiment yielded viable colonies and from the obtained biomass, we calculated that 57 doublings had occurred.
All nine of the F1 × F1 crosses from the acuta-c × acuta-p experiment yielded viable F2 progeny, with no apparent delay in reproduction [ 37].
In actual experiment, the viable spores may be genotyped by restriction enzyme digestion (Xu and He 2011), microarray-based SND typing (Kao et al. 2010), or genome sequencing.
Preliminary experiments employing viable HeLa cells are conducted in order to demonstrate the feasibility of the present design.
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