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Over the time frame of the experiment, viability of transduced cell remained at control values (>90%, data not shown) both in complete medium or after the 6h starvation.
For each experiment, viability counts (log CFU/mL) were plotted against time and expressed as the means of results from three separate assays.
It could be concluded that the arrest in reaction was not due to loss in viability, which remained high throughout the experiment (viability = 7.3 log (CFU/ml) after 144 h).
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In our experiments, viability remained constantly high (> 98.5 %) at both temperatures examined.
After 12 h exposure (time used in posterior experiments) viability was assessed using the crystal violet technique [ 18, 19].
In preliminary experiments, viability of U937 cells has been tested in the presence of PMA concentrations up to 200 nM and of WIN55212-2 concentrations up to 10 μM.
Also note that, unlike defined in the Methods (in the subsection headed "Fertility and fecundity experiments"), viability (=egg to adult survival, which is a proportion or percentage) is not the same as fertility.
Percentage cell death was determined by 20 μg ml−1 propidium iodide (PI) flow cytometry (FACSCalibur, Beckton Dickinson, Oxford, UK) counting 10 000 gated cells per condition wherever possible (in some experiments, viability was so low that only ∼4000 cells could be counted).
At the end of each experiment, cell viability was checked by use of an MTT assay, which was performed as described previously (De Ryck et al. 2014).
In the 2011 live plant array experiment, pollen viability differed significantly across subpopulations (Table 1a).
In the first experiment, cell viability was determined using an MTT assay.
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