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In the experiment, transient gastric irritation in the neonatal period could result in impaired brain gut axis and decrease some neuropeptides.
To perform the converse experiment, transient transfection of an expression vector containing the human SOX9 coding sequence was carried out to overexpress SOX9 in LX2 cells (Supporting Fig. 4D).
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In our experiments, transient capacitance was compensated, and output bandwidth filter was 3 kHz.
In other experiments, transient transfections were performed in 12-well plates.
In an independent series of experiments, transient intereference was achieved by using HIPK2-specific (HIPK2i) and universal negative control (UNC) siRNA.
In genetic rescue experiments, transient expression of Atg17-GFP fully rescued the autophagy defect of Atg17-null mutants (Fig. 4G).
In these experiments transient overexpression of BACE1 did not cause any alterations in the PCSK mRNA expression levels (data not shown).
Compared to stationary experiments, transients are much more sensitive to modifications of the catalyst acid/base properties.
A carefully designed wind tunnel experiment using transient liquid crystal technique is carried out to obtain surface Nusselt number distribution and pressure drop.
This experiment revealed transient changes in calcium influx within the tested cells (Figure 3C).
We also performed a knock-down experiment by transient transfection with an α-syntrophin-specific siRNA.
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