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Although in all experiments these compounds markedly increase ROS on complex I substrates, we must acknowledge substantial variation in the magnitude of ROS production from experiment to experiment, for example, compare figure 2 to figures 4 and 5.
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Reference variants were used to correct for experiment to experiment variation in yield.
The quality of the data obtained with this technology varies from experiment to experiment, and an efficient method for quality assessment is necessary to ensure their reliability.
The strains and methods used varied slightly from experiment to experiment; nevertheless similar results were obtained for the RRT analysis (Table 2).
Messenger RNA and protein expression levels were analyzed by analysis of variance (ANOVA) using experiment as a blocking factor to control for experiment-to-experiment variability.
To account for experiment-to-experiment variation in the expression levels of our different Pen-2 mutants, internal controls were included to allow proper normalization.
The reason for the experiment-to-experiment variation in PDL241 activity is unclear, but may reflect the health of human FcR positive cells in the engrafted mice.
One common isolate was used for each experiment to control for inter-experiment insect robustness.
Therefore, in our next experiment, we replicated this experiment to control for the movement of the cups.
A researcher is often able to identify one experiment of interest in the result set, and may use that experiment to look for similar experiments.
Two control samples will be run in each experiment to allow for normalization between experiments and periodical reproducibility experiments will be performed to guarantee correct measurements.
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