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ALAN TURING conceived the Turing machine as a thought experiment to define what can be mechanically computed.
In this issue of Structure, Lanouette and colleagues use a combination of computation and experiment to define a specificity motif for the lysine methyltransferase SMYD2.
Next we performed a time course experiment to define the time frame during reprogramming for which MBD3 is required.
The final elongation was 72°C for 5 min. We conducted a preliminary experiment to define optimal annealing temperature for each primer pair (shown in Table 3).
NCIS uses windows of low and randomly distributed sequence counts within each experiment to define the background and to separate it from the signal.
We performed a time-course microarray experiment to define the transcriptional response to carboplatin in vitro, and to correlate this with clinical outcome in epithelial ovarian cancer (EOC).
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However, transfection experiments (Figs. 6a and 6b) suggest promoter activity closer to the repeat tract and experiments to define the 5′ end of the CAG transcript(s) still need to be performed.
To gain insights into the differential responses of CNS and PNS neurons to reduced neurofibromin function, we designed a series of experiments to define the molecular mechanism(s) underlying the unique CNS neuronal sensitivity to Nf1 heterozygosity.
FOXP3-ΔN was thus used in subsequent experiments to define the DNA-binding specificity of FOXP3.
Mutagenesis experiments to define the role of different potential ets and other transcription factor binding sites in the regulation of IGF-1 expression are ongoing.
Past experiments to define the role of C6-aldehydes in plant defense responses were biased towards the exogenous application of the synthetic metabolites or the use of genetic manipulation of HPL expression levels in plant genotypes with intact ability to produce the competing AOS-derived metabolites.
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