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(33) Four-zone measurements were used at the beginning of each experiment to correct for imperfections of the optical components.
Hela cells were used as biological control in each set of experiment to correct any experimental variations.
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First we applied k, a correction factor often used in pooling experiments to correct the data for any unequal efficiencies in representing alleles [ 18] (see Methods section).
The second is intrinsic to pyrosequencing and can not be improved by increasing coverage; we therefore performed alternative types of experiments to correct the erroneous homopolymer calls found in the assembly.
In brief, amplified RNA was labeled with ULS Cy5 or Cy3 (Kreatech EA-006, Amsterdam) was pooled and co-hybridized to the microarray (each experiment was performed in dye swap fashion and controlled with self-self hybridization experiments to correct for dye-bias).
The pRL-SV40 was co-transfected in all experiments to correct the variations in transfection efficiency.
Microarray data are noisy overall (McCarroll and Altshuler 2007) and replicate experiments to correct these inaccuracies often difficult to repeat.
Briefly, normalization of the data was performed in GT using the subgrid Lowess algorithm with a 20% smoothing factor for all experiments to correct for systematic differences in data collection.
PCR data obtained in one of the normal kidney samples were arbitrarily chosen as control, and this sample was included in all PCR experiments to correct for possible interassay variations.
The present study aimed to test the utility of a novel behavioural experiment designed to correct distorted perception of sleep among patients diagnosed with primary insomnia.
To be able to normalize results between series of experiments and to correct for plate-to-plate variation, efflux to a standard preparation of HDL (50 μg protein/mL) was determined in triplicate.
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