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Participants were instructed to keep their right arm/hand and head motionless and the muscle relaxation was monitored throughout the entire experiment to check for involuntary movements.
A Fos-positive control and a negative control, obtained by omitting the primary antibody on a sample of two sections from each animal, were processed during each experiment to check for antibody specificity.
A 2 mV pulse was applied every 1 min throughout the experiment to check for the integrity of the epithelia.
In addition, long-term experimenters noted the results on data sheets during the experiment to check for interobserver-reliability (accordance of the results from data sheets and video coding: 100%).
Drug solutions were applied for 500 ms or 1 s every 3 min. The normalizing concentration of nicotine (75 μM) was applied several times to each cell during the course of an experiment to check for desensitization and/or rundown.
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Additionally, we have performed spike-in experiments to check for the accuracy of the miR-Q assays.
On the other hand, a stable association does not prove the validity of the modeling; it only suggests candidates for experiment to check.
After removal from the assembly, the capsules were weighed, pierced, dried and weighed again to determine the amount of fluid present during the experiment and to check for any fluid loss.
Additional experiments performed to check for toxicity included clinical chemistry and hematological blood parameters, as well as histological examination of a panel of organs for toxicity using hematoxylin and eosin (H&E) stain after killing the animals.
This control experiment was used to check for the presence of any artifactual edges between the disc and the background.
A calibration step of the experiment was used to check for PCR efficiency.
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