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Oligomycin was also employed in this experiment to block the F1Fo-ATPsynthase and monitoring the decay of ATP (corresponding to a rise in [Mg2+]c) in both LPC and HPC.
In an attempt to determine why T βRII expression alone induces transcriptional activation, we have performed experiment to block endogenous and exogenous TGF- β effect by a TGF- β receptor kinase inhibitor (SB-431542) (Halder et al, 2005).
(A ) Presynaptic action potential (top) and evoked IPSCs in 4 mM [Ca2+]o (bottom) before and after application of 300 µM of the low-affinity GABAA receptor antagonist TPMPA. 2 µM CGP55845 were added during the entire experiment to block GABAB receptors.
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NBQX (10 µM) and D-AP5 (20 µM) were present in all experiments to block glutamatergic EPSCs.
Such clusters then suggest binding sites or catalytic sites on the surface, and allosteric pathways internally [35], [37], [40], [41], [42], [43] which, in turn, efficiently guide experiments to block, separate, rewire, or mimic function [44] [54].
NMDG-based solutions were used in some experiments to block endogenous cation permeabilities.
QX314 (5 mM, Alomone labs) was included in the patch pipette in some experiments to block sodium channels intracellularly.
For example, the literature describes experiments to block expression of E6 in HPV-positive cancer cells by inhibiting viral transcription [ 43], by administering anti-sense constructs [ 46], and by administering RNAi targeting E6 [ 115] in cell culture.
Naloxone : These experiments sought to block TLR4 with naloxone treatment at the onset of the CT injury.
A similar behavior was observed when the neutrophils were incubated with soluble GPIbα prior to the experiment to competitively block Mac-1 receptors on the neutrophils.
Mossy fiber origin of recorded signals was routinely verified by frequency facilitation >400% and application of the group II metabotropic glutamate receptor agonist DCGIV (1 µM) at the end of the experiment, which had to block responses completely.
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