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To conduct an experiment with ChR, experimenters need to make the correct choices on the three main components to such an experiment: the expression system, the illumination source, and the ChR variant used.
In this experiment, the expression of WRKY45 in both genotypes was maintained normally, and the resistance induced by BTH was conserved (Fig. 2b).
BACKGROUND: In a time-course microarray experiment, the expression level for each gene is observed across a number of time-points in order to characterize the temporal trajectories of the gene-expression profiles.
Interestingly, in our experiment, the expression pattern of HO-1 resembled the pattern of CSE expression: it is localized to the media of the CA, was downregulated in CAD, and further significantly suppressed in sepsis.
In a control experiment, the expression of RanBP2 slightly increased under the same conditions, thereby indicating a specific down-regulation of Pias3 among the SUMO E3 ligases.
In the first experiment the expression of the genes encoding nine cytokines (interferon-γ, tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β), IL-2, IL-4, IL-5, IL-6, IL-8, and IL-10), as well as β-actin as the internal control, was assessed in triplicate after 2, 4, 8 and 12 h exposure to cultures of 95/1000.
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In several independent experiments, the expression of FGFR1 in control hESC H9 organoids showed similar patterns (Fig. 8a) as in the control iPSC organoids (Fig. 5a).
For both experiments, the expression values represent the percentage of transcript level normalised against the level for actA.
In these experiments, the expression of Wg and Hh was rotated 90° from their normal expression patterns.
In our experiments the expression of at least two let-7 members (let-7b and let-7f) increased during the 28 day time-course.
In our experiments, the expression of FLO11 was maximum over a wide concentration range of ammonium sulphate, i.e 25 µM–300 µM.
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