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To assess the duration of action of kisspeptin-mediated plasma extravasation formation, mice were treated i.d. with test agents and the experiment terminated after 7.5, 15 or 30 minutes.
The experiment terminated when all individuals died or reached metamorphosis.
It gave 100% knockdown against G.morsitans up to day 29 (when experiment terminated), 100% against G. pallidipes up to day 21 (experiment terminated) and up to day 15 with G.f. fuscipes.
Parasitaemia was measured at 48 h and 72 h by microscopy analysis of tail-cut blood samples and the experiment terminated at the later timepoint.
An experiment terminated in the arthritis phase (day 35) showed that Th17 cell numbers were lower in joints of E2-treated mice.
Pulse doses of N-dibutylnitrosamine(DBN), N-butyl-N- 4-hydroxybutyl)N-butyl-N- 4-hydroxybutyltyl-N-butyl-N- 4-hydroxybutylmine(BCPN) N-butyl-N- 4-hydroxybutylwere admiN-butyl-N- 4-hydroxybutylDF1 mice, anitrosamineriment terminated at one year of age.
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DOI: http://dx.doi.org/10.7554/eLife.04745.008 10.7554/eLiFigure45.009 Figure 3 figure supplement 1. Relative fitness in competition experiments terminated in mid-exponential phase ( lamB ).
DOI: http://dx.doi.org/10.7554/eLife.04745.009 10.7554/eLiFigure45.010 Figure 3 figure supplement 2. Relative fitness in competition experiments terminated in mid-exponential phase ( rplS syn ).
Most of the experiments terminate after selecting fewer than 10 features.
The experiments were terminated at 182h (7.5 days; experiment 1) and 120h (5 days; experiment 2).
In the CIA experiments presented, all mice developed arthritis by the time the experiment was terminated (100% incidence).
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