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For the remaining experiment, stimulation intensity (1120±489 µA) was kept 250 µA below the lower threshold for either phosphenes or skin sensations.
Importantly, video clips were not excluded from this experiment – stimulation was manipulated in such a way that the projector's bore was open just before the condition containing video stimulation and closed immediately after it.
In the INB and SB experiment stimulation trains lasted 500 ms. The duration was inspired by the minimal duration of stimulation trains used by Libet et al. [27] in order to produce perceivable sensory sensations.
The negative control for each experiment, stimulation of pDCs with medium alone, was assigned a value of 1, and all data are expressed as fold induction over the negative control.
In the NonREM Experiment stimulation was turned on after the subjects had attained at least 4 min of stable NonREM sleep for the first time after sleep onset, that is a time when sleep is expected to progress into slow wave sleep (SWS).
In the first step of the experiment, stimulation levels at three different dilutions of MC were measured in three healthy individuals, and statistically compared with one another.
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In our experiments, stimulation of the distal trunk of the vagus nerve showed anti-inflammatory properties, as reported by several other authors [11, 22 25], reducing peripheral cytokines in the current study.
For the three experiments, stimulation was applied at 90% of the RMT.
In other experiments, stimulation of cells with PMA did not alter interaction of AKIP 1A with PKAc or p65 (data not shown).
For these experiments, stimulation electrodes with 2 mm separation were positioned at the end of the muscle and signals were monitored at a region ∼2 mm distant from the electrodes.
In our experiments, stimulation with peptides exhibited in a significant proliferative effect on the osteoblast-like cells, especially within the early phase of cultivation.
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