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During the experiment, solution was heated and cooled using a Peltier element.
Few minutes after the preparation of experiment solution, 20 fingerlings (1.58 ± 0.02 g), i.e. fish in their exponential growth phase were carefully counted, weighed and placed into each tank at the stocking density of 1 g of fish/L.
The modeling conditions were chosen within the allowed settings of Mfold to approximate a microarray experiment: solution conditions of 1.0 M sodium concentration and no magnesium ion were used.
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Uneaten food and feces were siphoned upon renewal of experiment solutions.
Throughout the experiment, solutions were constantly aerated.
In "Solution methodology" and "Numerical experiments", solution methodology and conducted experimental results are presented, respectively.
It is possible that by experimenting, solutions can be identified that prevent some of the future losses that would otherwise occur.
They were "imagineers" - designers of gadgets, experiments, solutions.
For dose-response experiments, solutions contained (in mM): 95 NaCl, 5 KCl, 10 HEPES (pH 7.4), 10 D-Glucose, 2 MgCl2, 2 Cand2 and 45 mannitol (isotonic, 290 mannitol10% 30 mannitol (10% hypotonic, 261 mannitol1); 15 mannitol (20% hypotonic, 232 mmor·kg−1) or no mannitol (30% hypotonic, 203 mmol·kg−1).
For these experiments solutions were bubbled with argon before use.
During experiments, solutions in both chambers were continuously bubbled with air.
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