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JMC designed the experiment, sequenced and analysed the libraries and prepared the manuscript.
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PU.1 or Spi-B binding peaks were determined using replicate data from two experiments, sequenced by two independent sequencing core facilities with over 20 million reads per sample.
To ensure that initial Poly-Ribo-Seq experiments sequenced to an adequate depth and to potentially extend the catalogue of translated smORFs, we repeated the experiment but exclusively sequenced small polysomes.
The certainty of the above polynomial model can be estimated by the coefficient of determination, R 2. The experiment sequence was randomized to avoid systematic errors.
Thirty four of the 37 contigs which mapped to the preliminary experiment sequence belonged to the metacontig assembled from the full genome sequence and were in the correct order.
Ten clones in each experiment were sequenced and no mutations were detected.
The largest clone identified from our fingerprinting experiments was sequenced and assembled by the Baylor Genomics Center.
For each sample, DNA recovered from two independent Methyl Capture experiments was sequenced.
All positive products from Guthrie card experiments were sequenced to confirm the specificity of the product.
For these next experiments, I sequenced all pha-1 (ts) rescued F1's.
Most of the data (pathways, networks, microarray experiments, sequences, etc).
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