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During the experiment, saturation of magnetization of protons on the protein is transferred to protons of the bound ligand in roughly a 1/ r fashion.
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Binding affinity assays, qPCR experiments, saturation binding assays, and biodistribution studies were performed by ID.
In the second set of experiments, saturation of 99mTc-ZIGF1R:4551-GGGC was tested.
In the first set of experiments, saturation of I-IGF-1 binding was tested.
In accordance with competition experiments, saturation binding of GDP-less membranes with 0.3 to 10 µM [H]GDP displayed KD of 3.47 ± 0.03 µM and BMAX of 28 ± 3 fmol of binding sites per µg of protein.
Single-point ligand binding experiments and saturation binding experiments were conducted as described previously.
The monitoring experiment of saturation line in levee model is implemented on this platform.
In addition, Figs. 9 and 10 show Comparison between calculated and observed constant composition expansion (CCE) and CVD experiment, Liquid saturation, respectively.
A correction was made for the heat of dilution of the ligands, estimated from the peaks obtained from injections at the end of a given ITC experiment (following saturation).
The gain and offset were adjusted once and then used for one complete experiment, avoiding saturation.
To observe the correlation between the GR and anti-inflammatory potential of Rh1 combined with DEX, a parallel Western blot experiment and saturation binding analysis were performed.
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