In order to ensure maximal activation of the receptor in this complex immunoprecipitation experiment, saturating hormone concentrations of 100 nM were used, as usual for comparable experiments on insulin receptors [ 25- 30].
In experiments, saturated soils were exposed to various concentrations of the surfactant mixture for specified times, the slurries were centrifuged to separate the phases, the aqueous phases were extracted with 1,2-dichloroethane, and the residual homologs were derivatized with 3,5-dinitrobenzoyl chloride and analyzed by normal phase HPLC.
In these experiments, saturated cultures of SMR14350 were diluted 10,000-fold in M9 B1 0.1% glucose medium, grown to log phase shaking at 37°C, then GamGFP was induced by addition of 10 ng/ml doxycycline for 2 hr during shaking growth at 37°C prior to loading cells into the microfluidics chamber (time 0).
The calories I was getting from fat certainly increased during the experiment, and saturated fat made up a great part of the increase.
According to Iverson et al. (2011), the bed material in the experiment is saturated, and has a small cohesion, typically less than 400.
Three laboratory techniques were used to identify and quantify the chemical and hydrodynamic processes involved in strontium transport: batch experiments, stirred flow-through reactor experiments and saturated laboratory columns.
In further experiments, we saturated the pool of procollagen with [C]proline and added BFA to split the secretory pathway (shown schematically in Figure 4A).
In these experiments saturating or almost saturating concentrations of Mor protein (860 nM) were used, and each DNA probe assayed contained a Mor-only lane for comparison.
To further demonstrate that the increase in sPLA2-X was due to the enzymatic activity of the TGM2, we found that heat denaturing the TGM2 enzyme prior to the experiment, or saturating the active site of the enzyme with N-carbobenzoxy-Gln-Gly inhibited the activation of sPLA2-X (Figures 2F G).
Finally, we note that the sugar levels used in this experiment are saturating (0.2%).
