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As a control experiment, purified β-glucosidase was loaded on a SDS-PAGE after heating at 70 or 98°C, respectively and used for zymogram assay, indicating that tertiary conformation is impaired by heat, resulting in non-reversal refolding.
For this experiment, purified recombinant WRN488 1432 was acetylated by p300 in the presence or absence of acetyl CoA for 60 min at 30°C as described previously.
For this experiment, purified Foxp3-GFP+ OVA-specific iTreg cells that were generated in vitro from CD45.1+ mice were transferred into congenic CD45.2+ mice.
In the reciprocal experiment, purified GST and GST NO66 bound to glutathione resins were mixed with purified His-S-tagged Osterix protein in the presence of 10 mg/ml BSA in PBS and incubated at 4°C for 2 h.
At the beginning of this experiment, purified PP2A-B55 was mixed on ice with either buffer, nonradioactive pEndos, or nonradioactive thiophosphorylated Endos; then radioactive pCDKS substrate was added and the samples were incubated at 30°C starting at t = 0.
In this experiment, purified H. jecorina CEL7A and CEL6A were combined in various ratios with an RL-P37 background sample from which the cel7A and cel6A genes had been deleted (delta delta P37).
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In the present experiments, purified water, and water contaminated with a very small amount of surfactant (1-pentanol) were employed.
SZ carried out the microbial transformation experiments, purified all the metabolites, interpreted the spectroscopic data and prepared the preliminary draft of the manuscript.
In some experiments, purified T cells were diluted in RPMI (Roswell Park Memorial Institute) complete medium to achieve a concentration of 1 × 106 cells/mL.
In some experiments, purified B. pertussis LPS (10 µg/dose - produced by Instituto Butantan) was also combined with PspA5.
For the ligand screening experiments, purified recombinant vCD30 protein was amine-coupled to CM5 chips to a level of aprox.
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