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In this experiment, pool replicates were allelotyped on the same physical array (which holds two samples).
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To block out as many peptides as possible in the same experiment pooled polyclonal patient sera isolated from 20 different patients with suspected CD were used.
For further experiments, pool populations were maintained in liquid media containing G418 in above mentioned concentrations for the respective cell types.
While peptide Pool 10 scored positive in all three experiments, Pool 19 scored positive only once – therefore peptide 82, which occurred in these two pools, was initially not synthesized for further analyses.
The pooled data were not significantly different from the 3 13 ratio (ρ = 0.987), as shown by the χ test performed on the sum of the three experiments pooled for each class (Pooled χ) (Table 1).
Five wells per day and condition were used and two independent experiments pooled.
To prepare cells for experiments, pooled CNS samples were washed twice with 37% Percoll, resuspended in 30% Percoll, and layered over 70% Percoll.
In a subset of experiments pools of overlapping peptides spanning the HIV-1 proteins Gag, Pol, Env and Nef were used as antigens.
We failed to detect evidence of laterality having found no predominance of side bias in trial two of the experiments pooled (500 permutations; actual statistic = 11; p>0.75).
In all experiments, pools of reconstituted MEF cells were used.
For animal experiments, pooled Student's t-tests were performed to determine whether there was a difference between arthritic rats and matched control animals.
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