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We next manually picked 300 iPSC clones from the experiment plates at 8 dpt in order to catch pFind1-facilitated colonies.
In contrast, iPSC colonies appeared a few days earlier in the experiment plates that were transfected with OKS, pFind1 and pCAG-PBase.
The worms were transferred on the experiment plates 10 minutes before the start of recording to cut off the first minutes during which the worm may have responded to perturbations that could have occurred during transfer.
In a second experiment, plates were placed in containers, exposed to AA or volatiles for 5 d, and then inoculated with the fungal mycelium plug.
From the other set of experiment plates, we collected five fruiting bodies that had a D. discoideum phenotype and five fruiting bodies that had a D. purpureum phenotype.
In a third experiment, plates containing PDA medium were exposed to AA or volatiles for 24, 48, or 120 h, prior to inoculation.
Similar(53)
In a representative experiment, plate 1 of the LOPAC library was set-up for screening on day 1, and 3 other plates were set-up on the next day.
A standard curve of cDNA template (from a known sample) was run on each plate for each gene to allow for within experiment plate normalization.
Five independent experiments, each with different patient samples (fibroblasts and macrophage conditioned media) were performed with each individual experiment plated in triplicate to ensure repeatability.
Therefore, we detected and quantified E. coli O157 H7 by adopting single reactions, performed at the same time and in the same experiment plate.
For one of each control and experiment plate, we collected all of the fruiting bodies in 1 ml of KK2 buffer to count the number of spores that were produced.
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