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For in-vivo, mice administered with CELM significantly increased body weight throughout the experiment periods.
It was not clearly obvious that the animals showed markedly amended movements or changed behaviours inbetween the experiment periods.
Results indicated that the CELM enhanced the body weight of mice from beginning to end of experiment periods.
For all experiment periods, cells were maintained in osteogenic media, and media was changed every three days.
Every two days, the adipocytes were treated with 10, 50 and 100 μg/ml chloroform extract of L. multiflorum until the end of the experiment periods [10 days], without L. multiflorum extract considered as control cells.
Following hygiene regulations and to avoid animal infections, the mice stayed in their cages at all times, except during the experiment periods (within one day), and were taken care of by the animal center in the College of Medicine at National Taiwan University.
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The experiment period comprised 1 month and a half.
The experiment period lasted 84 days, preceded by an adaptation period of 28 days.
Daily rainfall during the dry season experiment period from February to May in 2011.
Access to facilitators for the enhanced sites was removed after the 12-month Experiment period.
Influent and effluent of the system were sampled and monitored continuously during the experiment period.
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