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Furthermore, we performed, at the end of every experiment of gene quantification, a determination of the melting point of the amplified fragments, to ensure that only a single fragment of DNA is amplified in each tube.
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Tarca, A. L., Romero, R. & Draghici, S. Analysis of microarray experiments of gene expression profiling.
Here, we critically evaluated the accuracy of flux balance analysis in two cancer types, clear cell renal cell carcinoma (ccRCC) and prostate adenocarcinoma, by comparison with large-scale experiments of gene essentiality in vitro.
Analysis of variance (one-way ANOVA) and multiple comparisons of differences between treatments (Duncan's multiple range test, p < 0.05, 0.01) were performed using SPSS for Windows version 11.5 (SPSS Inc .. XP performed the experiments of gene cloning and phenotype assays of transgenic plants.
We compared our theoretical predictions with the existing experiments of gene targeting using ODN in the literature.
All the experiments of gene expression profiling were authorized to be carried out by CapitalBio Corporation (Beijing, China).
Verification experiments of gene array findings included both real-time PCR analysis and in vitro metabolic tests.
Fold change is a commonly used measure in small laboratory experiments of gene expression; it is considered to be a natural measure for gene expression changes [ 29].
For the experiments of gene expression in sugarcane genotypes varying in sugar accumulation and sugarcane imposed with water deficit stress, RT-qPCR was performed in an ABI Prism® 7900HT Fast Real-Time PCR System (Applied Biosystems).
We have demonstrated the inequality and proportionality between Vg and Vip, through numerical evolution experiment of a gene network.
In our experiments, levels of gene expression were normalized using glyceraldehyde 3-phosphate dehydrogenase and β-actin.
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