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In another experiment of acute toxicity, different solvent extracts, namely, n-hexane, methylene chloride, ethyl acetate, and methanol extracts of F. vulgare upto 5.5 g/kg concentration, did not revealed any kind of toxicity in mice, LD50 being: 6.75, 11.0, 6.92, and 15 g/kg for n-hexane, methylene chloride, ethyl acetate, and methanol extracts, respectively [ 68].
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The experiments of acute toxicity in animals were performed in the animal house of Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan after the approval of departmental ethical committee.
One cow was excluded from the experiment because of acute spontaneous coliform mastitis after the first challenge.
They can be tightly controlled, can usually be undertaken quicker than ex vivo and in vivo experiments models of acute I/R injury, and can assess the direct effect of the novel cardioprotective therapy on the cardiomyocyte itself.
Hence loss of Aβ by diffusion may complicate interpretation of the effects of Aβ in experiments on acute slices from APP overexpressing mice.
The results of Experiment 3, acute effect of EPO on hemodynamic and cardiovascular function, are presented in Table 2.
Littermate Opn4Cre/+ R26iDTR/+ and WT mice were used in all subsequent experiments for acute ablation of mRGCs.
Also, it was shown that STIM protein knockdown did not affect the IP3 increase and subsequent Ca2+ release, as indicated by the Iosc amplitude responses evoked at the beginning of each experiment by acute application of one of the agonists studied.
In another part of experiments, acute effect of various doses of the Rosmarinus officinalis extract on blood glucose and serum insulin levels was studied in alloxan-induced diabetic rabbits.
The design of the UG was based on a previous study by Crockett, Clark, Tabibnia, Lieberman, and Robbins (2008), who used this task in a behavioral experiment studying the effects of acute tryptophan depletion.
The present experiments investigated the effects of acute methylphenidate and atomoxetine in male long Evans rats in the 5-choice serial reaction time (5CSRT) test that is hypothesized to serve as a model of vigilance and impulsivity behaviors associated with ADHD.
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