If not, how are the implications of this experiment limited?
For each column, different letters after the mean values represent significantly different means (Tukey's test, P < 0.05) aThis experiment was partly a replication of the UCPH experiment limited to five plants pot−1 (see Additional file 1 for details).
Notably, published in 2012, SMART-seq allowed for greater sensitivity and capturing of full-length transcripts, however cells had to be manually picked in that experiment limiting practical cell capture counts.
To test this alternative, the current experiment limited the blank interval between S1 and S2 to 300 ms considering visual iconic memory lasts approximately 250 ms after the stimuli's offset [67].
This could be related to the small number of fish tested in the experiment (limited by our collection permit) and to possible habituation to the animations.
Unreliability in the MTX dosing over blocks of the experiment limited our sample size (see methods) and therefore our power to detect QTL.
Moreover, as in most published studies, studying thawed plasma allowed to study all marker dosages simultaneously in the same experiment, limiting the inter-individual variability.
In contrast to the ELISPOT, the number of cells evaluated can be increased during the experiment, limited only by the total number of cells available.
Differences between the predicted and reported (e.g. literature, databases) gene relationships may be due to differences among the experiments considered (e.g. honey bee versus fruit fly) and insufficient or inaccurate information (e.g. small experiment, limited microarray platform).
